On-screen objects can be easily trapped and manipulated by selecting the "create trap" tool and clicking on the object. The HOTgui software creates an optical trap at that location. Up to 200 traps can be created in this fashion, which can be manipulated in three dimensions to form an ideal configuration for a given application. Traps can be moved by dragging them with the mouse, with preprogrammed paths, or by entering exact coordinates in the trap properties dialog box.
For each trap, you can select a number of trap properties to set trap position, intensity, charge and length. (see image below) The intensity control enables the user to vary the relative power of individual traps. The charge control allows the user to create optical vortices, which are ring-shaped traps that carry angular momentum. The length control can be used to create vertical line traps, which are traps that extend vertically into the sample.
The BioRyx 200 optical trapping system can operate with any of a variety of microscope objective lenses, of different magnification factors and working distances. The HOTgui software accomodates this ability; no recalibration or mechanical adjustments are necessary to switch between objective lenses.(see image below)
Images from the microscope can be recorded to disk as video or as still images for future review, analysis, or presentation. Utilities such as the scale bar and on-screen ruler allow for quick measurements. Recorded video can be used to set markers for assistance in configuring subsequent measurements.
The HOTgui software is intended for use with a BioRyx 200 optical
trapping system. Optional additional software is available. For
more information, see:
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These video clips demonstrate some of the capabilities of the HOTgui software. Click on the image to activate the movie (WMV). Videos appear best in full-screen mode. More videos can be found on the Demo Video Page.
- Point-and-click trapping of all on-screen objects
- Using three-dimensional real-time trapping to move one trapped object over another
- Forming a group of objects and moving them together in real time
- Defining multiple paths that move trapped objects in three dimensions
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